永利澳门

永利澳门:季泉江

时间:2018-09-04浏览:19603设置

季泉江课题组介绍


Principal Investigator


Quanjiang Ji (季泉江), Assistant Professor, PI

Address: 393 Huaxia Middle Road, Pudong, Shanghai, China, 200120

Email: quanjiangji@shanghaitech.edu.cn


Assistant Professor, ShanghaiTech University, 2016-current;

Postdoc., University of California, Berkeley (Advisor: Prof. Michelle Chang), 2014-2016;

Ph.D., University of Chicago (Advisor: Prof. Chuan He), 2009-2014;

B.S., Nanjing University, 2005-2009.


Awards:

NSFC Excellent Young Scholar,2019;
Shanghai Science and Technology Committee Rising-Star Program,2019;
Camille and Henry Dreyfus Postdoctoral Fellowship, 2015;
Chinese Government Award for Outstanding Self-Financed Students Abroad, 2013;
The Everett E Gilbert Memorial Prize, 2012.


Research


The emergence of drug-resistant human pathogens has posed a severe public health crisis worldwide. To counter infections caused by major human pathogens, we aim to create novel genome editing tools, address fundamental infection and drug-resistant mechanisms, and develop new therapeutic means with the utilization of multiple approaches (chemical, biological, and engineering).

Genome editing in human pathogens: Genetics is the key means to study bacterial physiology. However, traditional genetic manipulation methods in major human pathogens remain as time-consuming and laborious endeavors. We have created rapid and highly efficient genetic manipulation tools in multiple major human pathogens, including Staphylococcus aureus (JACS, 2017; Chem Sci, 2018; Chem Sci, 2020), Pseudomonas aeruginosa (iScience, 2018), Klebsiella pneumoniae (Appl Environ Microbiol, 2018; Antimicrob Agents Chemother, 2019), and Acinetobacter baumannii (Cell Chem Biol, 2019) by engineering the powerful CRISPR/Cas9 genome editing technology and deaminase-mediated base editing systems. These tools have been requested and utilized by numerous research groups worldwide and are available in Addgene (http://www.addgene.org/Quanjiang_Ji/).

We utilize protein engineering and synthetic biology approaches to develop genome-wide screening tools and aim to construct label-free gene-knockout libraries in multiple human pathogens. The development of these tools will advance fundamental physiology studies as well as novel drug-target exploration.

We study fundamental DNA recognition and cleavage mechanisms of CRISPR systems (PLoS Biol, 2019; Nat Catal, 2020). We design, engineer, and functionalize CRISPR systems for diverse applications, in particular in the study of infectious diseases.

Fundamental infection and drug-resistant mechanisms: We study basic infection and drug-resistant mechanisms in major human pathogens by taking advantages of structural biology and genome editing approaches (PNAS, 2018; Mol Microbiol, 2018). Recently, we focused on elucidating the molecular mechanisms of nicotianamine-like metallophore-mediated transition metal acquisition processes as well as biofilm-formation mechanisms of several cell surface proteins in human pathogens. We are also interested in small-molecule signaling and regulatory pathways that affect bacterial pathogenesis and drug resistance.

Therapeutic means against infections: We develop therapeutic antibodies and small molecules targeting key virulence or drug-resistant proteins, in particular the extracellular proteins that play vital roles in bacterial metal acquisition and biofilm formation. We aim to identify new drug targets using the mutant libraries we are currently constructing and screen effective antibodies or small molecules against them.


Publications


Book Chapters

1. Chen, W. & Ji, Q. (2020) Genetic manipulation of MRSA using CRISPR/Cas9 technology. Methods in Molecular Biology 2069:113-124.


Research Articles and Reviews

2020

18. Zhang, Y., Zhang, H., Xu, X., Wang, Yuj, Chen, W., Wang, Ya., Wu, Z., Tang, N., Wang, Yu, Zhao, S., Gan, J.*, Ji, Q.* (2020) Catalytic-state structure and engineering of Streptococcus thermophilus Cas9. Nature Catalysis DOI: 10.1038/s41929-020-00506-9.


17. Yu, H.#, Wu, Z.#, Chen, X., Ji, Q.*, Tao, S.* (2020) CRISPR-CBEI: a designing and analyzing tool kit for cytosine base editor-mediated gene inactivation. mSystems 5: e00350-20.


16. Wang, Y.*, Wang, Z., Ji, Q.* (2020) CRISPR-Cas9-based genome editing and cytidine base editing in Acinetobacter baumanniiSTAR Protocols DOI: 10.1016/j.xpro.2020.100025.


15. Pi, Y., Chen, W., Ji, Q.* (2020) Structural basis of Staphylococcus aureus surface protein SdrC. Biochemistry 59: 1465-1469.


14. Zhang, Y., Zhang, H., Wang, Z., Wu, Z., Wang, Y., Tang, N., Xu, X., Zhao, S., Chen, W.*, Ji, Q.* (2020) Programmable adenine deamination in bacteria using a Cas9-adenine-deaminase fusion. Chemical Science 11: 1657-1664.


13. Wu, Z., Wang, Y., Zhang, Y., Chen, W., Wang, Y., Ji, Q.* (2020) Strategies for developing CRISPR-based gene editing methods in bacteria. Small Methods 4: 1900560.


2019

12. Chen, W., Zhang, H., Zhang, Y., Wang, Y., Gan, J.*, Ji, Q.* (2019) Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. PLoS Biology 17: e3000496.


11. Wang, Y., Wang, Z., Chen, Y., Hua, X., Yu, Y., Ji, Q.* (2019) A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii toward the understanding of H2O2-sensing mechanism of OxyR. Cell Chemical Biology 26: 1732-42. 


10. Zhang, Y., Sun, X., Qian, Y., Yi, H., Song, K., Zhu, H., Zonta, F., Chen, W., Ji, Q., Miersch, S, Sidhu, S.S.*, Wu, D.* (2019) A potent anti-SpuE antibodyallosterically inhibits type III secretion system and attenuates virulence of Pseudomonas aeruginosa. Journal of Molecular Biology 431:4882-4896.


9. Fu, T., Liu, L., Yang, Q.L., Wang, Y., Xu, P., Zhang, L., Liu, S., Dai, Q., Ji, Q., Xu, G.L., He, C., Luo, C.*, Zhang, L.* (2019) Thymine DNA glycosylase recognizes the geometry alteration of minor grooves induced by 5-formylcytosine and 5-carboxylcytosine. Chemical Science 10: 7407-17.


8. He, T., Wang, R., Liu, D., Walsh, T.R., Zhang, R., Lv, Y., Ke, Y., Ji, Q., Wei, R., Liu, Z., Shen, Y., Wang, G., Sun, L., Lei, L., Lv, Z., Li, Y., Pang, M., Wang, L., Sun, Q., Fu, Y., Song, H., Hao, Y., Shen, Z., Wang, S., Chen, G., Wu, C., Shen, J., Wang, Y. (2019) Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans. Nature Microbiology 4: 1450-6.


7. Sun, Q. #, Wang, Y. #, Dong, N., Shen, L., Zhou, H., Hu, Y., Gu, D., Chen, S., Zhang, R.*, Ji, Q.* (2019) Application of CRISPR/Cas9-based genome editing in studying the mechanism of pandrug resistance in Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 63: e00113-19.


2018

6. Wang, Y., Wang, S., Chen, W., Song, L., Shen, Z., Yu, F., Li, M., Ji, Q.*(2018) Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Applied and Environmental Microbiology 84: e01834-18.


5. Chen, W., Zhang, Y., Zhang, Y., Pi, Y., Gu, T., Song, L., Wang, Y., Ji, Q.* (2018) CRISPR/Cas9-based genome editing in Pseudomonas aeruginosa and cytidine deaminase-mediated base editing in Pseudomonas species. iScience 6: 222-31.


4. Wei, W.#, Zhang, Y.#, Gao, R., Li, J., Xu, Y., Wang, S., Ji, Q.*, Feng, Y.* (2018) Crystal structure and acetylation of BioQ suggests a novel regulatory switch for biotin biosynthesis in Mycobacterium smegmatis. Molecular Microbiology 109: 642-62.


3. Song, L., Zhang, Y., Chen, W., Gu, T., Zhang, S.Y., Ji, Q.* (2018) Mechanistic insights into staphylopine-mediated metal acquisition.  PNAS 115: 3942-7.


2. Gu, T.#, Zhao, S.#, Pi, Y., Chen, W., Chen, C., Liu, Q., Li, M., Han, D.*, Ji, Q.* (2018) Highly efficient base editing in Staphylococcus aureus using an engineered CRISPR RNA-guided cytidine deaminase. Chemical Science 9: 3248-53.


2017

1. Chen, W., Zhang, Y., Yeo, W.S., Bae, T., Ji, Q.* (2017) Rapid and efficient genome editing in Staphylococcus aureus by using an engineered CRISPR/Cas9 system. JACS 139: 3790-5.


Patents


8. 季泉江、王宇。一种用于鲍曼不动杆菌胞嘧啶碱基编辑质粒及其应用。申请号:201910644444.1
7. 季泉江、王宇。双质粒系统及其应用。申请号:201910644324.1
6. 季泉江、王宇。一种用于肺炎克雷伯菌基因编辑的双质粒系统。申请号:201811039504.9
5. 季泉江、王宇。一种肺炎克雷伯菌基因编辑的表达载体。申请号:201811039489.8
4. 季泉江、陈未中。一种pnCasPA-BEC质粒及其应用。申请号:201810767194.6
3. 季泉江、陈未中。一种pCasPA/pACRISPR双质粒系统及其应用。申请号:201810766759.9
2. 季泉江、顾桐年。一种pnCasSA-BEC质粒及其应用。申请号:201810169946.9
1. 季泉江、陈未中。一种pCasSA质粒及其应用。授权号:ZL201611255504.3


Group Activities


Tianmu Lake (10/2019)


Current Group Members


Weizhong Chen(陈未中)

Research associate professor
Research associate professor, ShanghaiTech University, 2020-Current;                              Research assistant professor, ShanghaiTech University, 2018-2020                           Postdoc., ShanghaiTech University, 2016-2018;                                                                    Ph.D., University of Science and Technology of China, 2009-2015;                                            B.S., University of Science and Technology of China, 2005-2009.

Email: chenwzh@shanghaitech.edu.cn

Zhaowei Wu 吴兆韡)

Postdoc
Postdoc., ShanghaiTech University, 2018-Current;
Ph.D., Northwest A&F University, 2014-2018;
B.S., Northwest A&F University, 2010-2014.

Email: wuzw1@shanghaitech.edu.cn


Yifei Zhang (张翼飞)

Graduate student
Graduate student, ShanghaiTech University, 2016-Current;
B.S., Zhengzhou University, 2012-2016.

Email: zhangyf1@shanghaitech.edu.cn



Ya Zhang (张雅)

Graduate student
Graduate student, ShanghaiTech University, 2017-Current;                                                          B.S., Southwest University, 2013-2017.

Email: zhangya@shanghaitech.edu.cn

Zhipeng Wang(王志鹏)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;                                                  B.S., Zhengzhou University, 2014-2018.

Email: wangzhp@shanghaitech.edu.cn


Yujue Wang(王玉珏)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;
B.S., Shandong University, 2014-2018.

Email: wangyj6@shanghaitech.edu.cn

Hongyuan Zhang (张洪源)

Graduate student
Graduate student, ShanghaiTech University, 2018-Current;
B.S., Shandong University, 2014-2018.

Email: zhanghy2@shanghaitech.edu.cn

Na Tang(汤娜)

Graduate student
Graduate student, ShanghaiTech University, 2019-Current;
B.S., China Pharmaceutical University, 2015-2019.

Email: tangna@shanghaitech.edu.cn

Yannan Wang(王艳男)

Graduate student
Graduate student, ShanghaiTech University, 2019-Current;
B.S., Wuhan University of Technology, 2015-2019.

Email: wangyn5@shanghaitech.edu.cn

Chang Liu (刘畅)

Undergraduate student
Undergraduate, ShanghaiTech University, 2017-Current;

Email: liuchang2@shanghaitech.edu.cn


Alumni







Liqiang Song (宋立强)

Postdoc   2016-2018
Current: UT Health Science Center    (Postdoc)





Yu Wang (王宇)

Postdoc   2017-2019
Current: Jiangxi Agricultural University (Associate Professor) 





Yani Zhao (赵亚妮)

Undergraduate   2017-2019
Current: UNC at Chapel Hill                       (Ph.D. candidate)


Tongnian Gu (顾桐年)

Ph.D.   2015-2020
Current: Institute of Biophysics, CAS (Postdoc)


Yishuang Pi (皮义双)

M.S.   2017-2020
Current: Viva Biotech                           (Research Scientist)







返回原图
/

永利澳门-永利澳门娱乐场3229